An ultra-high-density protein microarray for high throughput single-tier serological detection of Lyme disease.

ABSTRACT: Current serological immunoassays have inherent limitations for certain infectious diseases such as Lyme disease, a bacterial infection caused by Borrelia burgdorferi in North America. Here we report a novel method of manufacturing high-density multiplexed protein microarrays with the capacity to detect low levels of antibodies accurately from small blood volumes in a fully automated system. A panel of multiple serological markers for Lyme disease are measured using a protein microarray system, Lyme Immunochip, in a single step but interpreted adhering to the standard two-tiered testing algorithm (enzyme immunoassay followed by Western blot). Furthermore, an enhanced IgM assay was supplemented to improve the test's detection sensitivity for early Lyme disease. With a training cohort (n = 40

SUBMITTER: Jayaraman V 

PROVIDER: S-EPMC7581523 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature