Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon.

ABSTRACT: Genetic introgression of escaped farmed Atlantic salmon (Salmo salar) into wild populations is a major environmental concern for the salmon aquaculture industry. Using sterile fish in commercial aquaculture operations is, therefore, a sustainable strategy for bio-containment. So far, the only commercially used methodology for producing sterile fish is triploidization. However, triploid fish are less robust. A novel approach in which to achieve sterility is to produce germ cell-free salmon, which can be accomplished by knocking out the dead-end (dnd) gene using CRISPR-Cas9. The lack of germ cells in the resulting dnd crispants, thus, prevents reproduction and inhibits subsequent large-scale production of sterile fish. Here, we report a rescue approach for producing germ cells in Atlantic sa

SUBMITTER: Guralp H 

PROVIDER: S-EPMC7581530 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature