Infarct Collagen Topography Regulates Fibroblast Fate via p38-Yes-Associated Protein Transcriptional Enhanced Associate Domain Signals.

ABSTRACT:

Rationale

Myocardial infarction causes spatial variation in collagen organization and phenotypic diversity in fibroblasts, which regulate the heart's ECM (extracellular matrix). The relationship between collagen structure and fibroblast phenotype is poorly understood but could provide insights regarding the mechanistic basis for myofibroblast heterogeneity in the injured heart.

Objective

To investigate the role of collagen organization in cardiac fibroblast fate determination.

Methods and results

Biomimetic topographies were nanofabricated to recapitulate differential collagen organization in the infarcted mouse heart. Here, adult cardiac fibroblasts were freshly isolated and cultured on ECM topographical mimetics for 72 hours. Aligned mimetics caused cardiac fibrobla

SUBMITTER: Bugg D 

PROVIDER: S-EPMC7581544 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature