Characterization of rare spindle and root cell transcriptional profiles in the stria vascularis of the adult mouse cochlea.

ABSTRACT: The stria vascularis (SV) in the cochlea generates and maintains the endocochlear potential, thereby playing a pivotal role in normal hearing. Knowing transcriptional profiles and gene regulatory networks of SV cell types establishes a basis for studying the mechanism underlying SV-related hearing loss. While we have previously characterized the expression profiles of major SV cell types in the adult mouse, transcriptional profiles of rare SV cell types remained elusive due to the limitation of cell capture in single-cell RNA-Seq. The role of these rare cell types in the homeostatic function of the adult SV remain largely undefined. In this study, we performed single-nucleus RNA-Seq on the adult mouse SV in conjunction with sample preservation treatments during the isolation steps. We dist

SUBMITTER: Gu S 

PROVIDER: S-EPMC7581811 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature