The Site-Specific Recombination System of the Escherichia coli Bacteriophage Φ24_B.

ABSTRACT: Stx bacteriophages are members of the lambdoid group of phages and are responsible for Shiga toxin (Stx) production and the dissemination of Shiga toxin genes (stx) across shigatoxigenic Escherichia coli (STEC). These toxigenic bacteriophage hosts can cause life-threatening illnesses, and Stx is the virulence determinant responsible for the severe nature of infection with enterohemorrhagic E. coli, a subset of pathogenic STEC. Stx phages are temperate, and in the present study, the identification of what is actually required for Stx phage Φ24_B and bacterial DNA recombination was tested using both in vitro and in situ recombination assays. It is well established that phage λ, which underpins most of what we understand about lambdoid phage biolog

SUBMITTER: Mohaisen MR 

PROVIDER: S-EPMC7581858 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature