NDRG2 regulates adherens junction integrity to restrict colitis and tumourigenesis.

ABSTRACT:

Background

Paracellular barriers play an important role in the pathogenesis of Inflammatory bowel disease (IBD) and maintain gut homeostasis. N-myc downstream-regulated gene 2 (NDRG2) has been reported to be a tumour suppressor gene and to inhibit colorectal cancer metastasis. However, whether NDRG2 affects colitis initiation and colitis-associated colorectal cancer is unclear.

Methods

Intestine-specific Ndrg2 deficiency mice (Ndrg2^ΔIEC) were subjected to DSS- or TNBS-induced colitis, and AOM-DSS-induced colitis-associated tumour. HT29 cells, Caco2 cells, primary intestinal epithelial cells (IECs) from Ndrg2^ΔIEC mice, mouse embryo fibroblasts (MEFs) from systemic Ndrg2 knockout mice, HEK293 cells and human UC and DC specimens were used to investigate ND

SUBMITTER: Wei M 

PROVIDER: S-EPMC7581885 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature