Mode of induction of platelet-derived extracellular vesicles is a critical determinant of their phenotype and function.

ABSTRACT: Platelet-derived extracellular vesicles (PDEVs) are the most abundant amongst all types of EVs in the circulation. However, the mechanisms leading to PDEVs release, their role in coagulation and phenotypic composition are poorly understood. PDEVs from washed platelets were generated using different stimuli and were characterised using nanoparticle tracking analysis. Procoagulant properties were evaluated by fluorescence flow cytometry and calibrated automated thrombography. EVs from plasma were isolated and concentrated using a novel protocol involving a combination of size exclusion chromatography and differential centrifugation, which produces pure and concentrated EVs. Agonist stimulation enhanced PDEV release, but did not alter the average size of EVs compared to those produced by unst

SUBMITTER: Ferreira PM 

PROVIDER: S-EPMC7582134 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature