A Survey of Barley PIP Aquaporin Ionic Conductance Reveals Ca²⁺-Sensitive HvPIP2;8 Na⁺ and K⁺ Conductance.

ABSTRACT: Some plasma membrane intrinsic protein (PIP) aquaporins can facilitate ion transport. Here we report that one of the 12 barley PIPs (PIP1 and PIP2) tested, HvPIP2;8, facilitated cation transport when expressed in Xenopus laevis oocytes. HvPIP2;8-associated ion currents were detected with Na⁺ and K⁺, but not Cs⁺, Rb⁺, or Li⁺, and was inhibited by Ba²⁺, Ca²⁺, and Cd²⁺ and to a lesser extent Mg²⁺, which also interacted with Ca²⁺. Currents were reduced in the presence of K⁺, Cs⁺, Rb⁺, or Li⁺ relative to Na⁺ alone. Five HvPIP1 isoforms co-expressed with HvPIP2;8 inhibited the ion conductance relative to <

SUBMITTER: Tran STH 

PROVIDER: S-EPMC7582361 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature