Fast, large area multiphoton exoscope (FLAME) for macroscopic imaging with microscopic resolution of human skin.

ABSTRACT: We introduce a compact, fast large area multiphoton exoscope (FLAME) system with enhanced molecular contrast for macroscopic imaging of human skin with microscopic resolution. A versatile imaging platform, FLAME combines optical and mechanical scanning mechanisms with deep learning image restoration to produce depth-resolved images that encompass sub-mm² to cm² scale areas of tissue within minutes and provide means for a comprehensive analysis of live or resected thick human skin tissue. The FLAME imaging platform, which expands on a design recently introduced by our group, also features time-resolved single photon counting detection to uniquely allow fast discrimination and 3D virtual staining of melanin. We demonstrate its performance and utility by fast ex vivo and

SUBMITTER: Fast A 

PROVIDER: S-EPMC7582965 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature