Ontology highlight
ABSTRACT:
SUBMITTER: Ding L
PROVIDER: S-EPMC7582981 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
International journal of molecular sciences 20200926 19
<i>Kluyveromyces marxianus</i> (<i>K. marxianus</i>) is an increasingly popular industrially relevant yeast. It is known to possess a highly efficient non-homologous end joining (NHEJ) pathway that promotes random integration of non-homologous DNA fragments into its genome. The nature of the integration events was traditionally analyzed by Southern blot hybridization. However, the precise DNA sequence at the insertion sites were not fully explored. We transformed a PCR product of the <i>Saccharo ...[more]