Immunopharmacology and Quantitative Analysis of Tyrosine Kinase Signaling.

ABSTRACT: In this article we describe the use of pharmacological and genetic tools coupled with immunoblotting (Western blotting) and targeted mass spectrometry to quantify immune signaling and cell activation mediated by tyrosine kinases. Transfer of the ATP γ phosphate to a protein tyrosine residue activates signaling cascades regulating the differentiation, survival, and effector functions of all cells, with unique roles in immune antigen receptor, polarization, and other signaling pathways. Defining the substrates and scaffolding interactions of tyrosine kinases is critical for revealing and therapeutically manipulating mechanisms of immune regulation. Quantitative analysis of the amplitude and kinetics of these effects is becoming ever more accessible experimentally and increasingly important f

SUBMITTER: Brian BF 

PROVIDER: S-EPMC7583487 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature