Lipid Composition Affects the Efficiency in the Functional Reconstitution of the Cytochrome c Oxidase.

ABSTRACT: The transmembrane protein cytochrome c oxidase (CcO) is the terminal oxidase in the respiratory chain of many aerobic organisms and catalyzes the reduction of dioxygen to water. This process maintains an electrochemical proton gradient across the membrane hosting the oxidase. CcO is a well-established model enzyme in bioenergetics to study the proton-coupled electron transfer reactions and protonation dynamics involved in these processes. Its catalytic mechanism is subject to ongoing intense research. Previous research, however, was mainly focused on the turnover of oxygen and electrons in CcO, while studies reporting proton turnover rates of CcO, that is the rate of proton uptake by the enzyme, are scarce. Here, we reconstitute CcO from R. sphaeroides into liposomes containing a pH sensit

SUBMITTER: Hugentobler KG 

PROVIDER: S-EPMC7583929 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature