Cigarette smoke extract stimulates bronchial epithelial cells to undergo a SUMOylation turnover.

ABSTRACT:

Background

Chronic obstructive pulmonary disease (COPD) characterized by the airway and lung inflammation, is a leading cause of morbidity and mortality worldwide, especially among smokers over 40 years of age and individuals exposed to biomass smoke. Although the detailed mechanisms of this disease remain elusive, there is feasible evidence that protein posttranslational modifications (PTMs) may play a role in its pathoetiology. We thus conducted studies to dissect the effect of cigarette smoke extracts (CSE) on the change of SUMOylated substrates in human bronchial epithelial cells (HBEs).

Methods

Samples were collected in HBEs with or without 24 h of CSE insult and then subjected to Western-blot and LC-MS/MS analysis. Subsequently, bioinformatic tools were used to analyze

SUBMITTER: Zhou H 

PROVIDER: S-EPMC7584069 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature