Microbial upgrading of acetate into 2,3-butanediol and acetoin by E. coli W.

ABSTRACT:

Background

Acetate is an abundant carbon source and its use as an alternative feedstock has great potential for the production of fuel and platform chemicals. Acetoin and 2,3-butanediol represent two of these potential platform chemicals.

Results

The aim of this study was to produce 2,3-butanediol and acetoin from acetate in Escherichia coli W. The key strategies to achieve this goal were: strain engineering, in detail the deletion of mixed-acid fermentation pathways E. coli W ΔldhA ΔadhE Δpta ΔfrdA 445_Ediss and the development of a new defined medium containing five amino acids and seven vitamins. Stepwise reduction of the media additives further revealed that diol production from acetate is mediated by the availability of aspartat

SUBMITTER: Novak K 

PROVIDER: S-EPMC7584085 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature