Development of a protein microarray-based diagnostic chip mimicking the skin prick test for allergy diagnosis.

ABSTRACT: Protein microarrays have been successfully used for detection of allergen-specific IgE in patient sera. Here, we demonstrate proof-of-concept of a solid-phase technique coupling the high-throughput potential of protein microarrays with the biologically relevant readout provided by IgE reporter cells, creating a novel allergic sensitization detection system. Three proteins (κ-casein, timothy grass pollen extract, polyclonal anti-human IgE) were printed onto three different polymer-coated surfaces (aldehyde-, epoxy- and NHS ester-coated). ToF-SIMs analysis was performed to assess printed protein stability and retention during washing steps. NFAT-DsRed rat basophil leukemia cell attachment and retention during washing steps was assessed after treatment with various extracellular matrix protei

SUBMITTER: Kalli M 

PROVIDER: S-EPMC7584649 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature