Multicenter assessment of microbial community profiling using 16S rRNA gene sequencing and shotgun metagenomic sequencing.

ABSTRACT:

Introduction

Microbiome research based on high-throughput sequencing has grown exponentially in recent years, but methodological variations can easily undermine the reproducibility across studies.

Objectives

To systematically evaluate the comparability of sequencing results of 16S rRNA gene sequencing (16Ss)- and shotgun metagenomic sequencing (SMs)-based microbial community profiling in laboratories under routine conditions.

Methods

We designed a multicenter study across 35 participating laboratories in China using designed mock communities and homogenized fecal samples.

Results

A wide range of practices and approaches was reported by the participating laboratories. The observed microbial compositions of the mock communities in 46.2% (12/26) of the 16Ss and 82

SUBMITTER: Han D 

PROVIDER: S-EPMC7584675 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature