Role of Long Noncoding RNA Gas5 in Cocaine Action.

ABSTRACT:

Background

Long noncoding RNAs (lncRNAs) are a class of transcribed RNA molecules greater than 200 nucleotides in length. Although lncRNAs do not encode proteins, they play numerous functional roles in gene expression regulation. lncRNAs are notably abundant in brain; however, their neural functions remain largely unknown.

Methods

We examined the expression of the lncRNA Gas5 in nucleus accumbens (NAc), a key brain reward region, of adult male mice after cocaine administration. We then performed viral-mediated overexpression of Gas5 in NAc neurons to determine its role in addiction-related behaviors. We also carried out RNA sequencing to investigate Gas5-mediated transcriptomic changes.

Results

We demonstrated that repeated short-term or long-term cocaine administrati

SUBMITTER: Xu H 

PROVIDER: S-EPMC7584769 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature