Lysozyme Resistance in Clostridioides difficile Is Dependent on Two Peptidoglycan Deacetylases.

ABSTRACT: Clostridioides (Clostridium) difficile is a major cause of hospital-acquired infections leading to antibiotic-associated diarrhea. C. difficile exhibits a very high level of resistance to lysozyme. Bacteria commonly resist lysozyme through modification of the cell wall. In C. difficile, σ^V is required for lysozyme resistance, and σ^V is activated in response to lysozyme. Once activated, σ^V, encoded by csfV, directs transcription of genes necessary for lysozyme resistance. Here, we analyze the contribution of individual genes in the σ^V regulon to lysozyme resistance. Using CRISPR-Cas9-mediated mutagenesis we constructed in-frame deletions of single genes in the csfV operon. We find that pdaV,

SUBMITTER: Kaus GM 

PROVIDER: S-EPMC7585060 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature