The Role of YggS in Vitamin B₆ Homeostasis in Salmonella enterica Is Informed by Heterologous Expression of Yeast SNZ3.

ABSTRACT: YggS (COG0325) is a pyridoxal 5'-phosphate (PLP)-binding protein proposed to be involved in homeostasis of B₆ vitamers. In Salmonella enterica, lack of yggS resulted in phenotypes that were distinct and others that were similar to those of a yggS mutant of Escherichia coli Like other organisms, yggS mutants of S. enterica accumulate endogenous pyridoxine 5'-phosphate (PNP). Data herein show that strains lacking YggS accumulated ∼10-fold more PLP in growth medium than a parental strain. The deoxyxylulose 5-phosphate-dependent biosynthetic pathway for PLP and the PNP/pyridoxamine 5'-phosphate (PMP) oxidase credited with interconverting B₆ vitamers were replaced with a single PLP synthase from Saccharomyces cerevisiae The

SUBMITTER: Vu HN 

PROVIDER: S-EPMC7585061 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature