Insulin sensitivity in long-lived growth hormone-releasing hormone knockout mice.

ABSTRACT: Our previous studies showed that loss-of-function mutation of growth hormone releasing hormone (GHRH) results in increased longevity and enhanced insulin sensitivity in mice. However, the details of improved insulin action and tissue-specific insulin signaling are largely unknown in this healthy-aging mouse model. We conducted hyperinsulinemic-euglycemic clamp to investigate mechanisms underlying enhanced insulin sensitivity in growth hormone (GH) deficient mice. Further, we assessed in vivo tissue-specific insulin activity via activation of PI3K-AKT and MAPK-ERK1/2 cascades using western blot. Clamp results showed that the glucose infusion rate required for maintaining euglycemia was much higher in GHRH^-/- mice compared to WT controls. Insulin-mediated glucose production

SUBMITTER: Zhang F 

PROVIDER: S-EPMC7585079 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature