IL-32 induces epithelial-mesenchymal transition by triggering endoplasmic reticulum stress in A549 cells.

ABSTRACT:

Background

Epithelial-mesenchymal transition (EMT) is a key process in the onset and development of idiopathic pulmonary fibrosis (IPF) with unclear mechanisms. Our previous studies found that bleomycin and tunicamycin could induce ER stress and consequently trigger EMT accompanying with IL-32 overexpression. This study was aimed to investigate the effects of IL-32 on EMT and ER stress to elucidate the pathogenesis of IPF.

Methods

Human lung adenocarcinoma A549 cells were treated with recombinant human (rh)IL-32, IL-32 siRNA and EMT inducer tunicamycin, or 4-phenylbutyric acid (4-PBA), respectively. Then the cell morphology was observed and the expression of ER-related markers and EMT-related markers were detected by RT-qPCR or western blotting.

Results

Stimulation of

SUBMITTER: Gong L 

PROVIDER: S-EPMC7585222 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature