Pacific Biosciences assembly with Hi-C mapping generates an improved, chromosome-level goose genome.

ABSTRACT:

Background

The domestic goose is an economically important and scientifically valuable waterfowl; however, a lack of high-quality genomic data has hindered research concerning its genome, genetics, and breeding. As domestic geese breeds derive from both the swan goose (Anser cygnoides) and the graylag goose (Anser anser), we selected a female Tianfu goose for genome sequencing. We generated a chromosome-level goose genome assembly by adopting a hybrid de novo assembly approach that combined Pacific Biosciences single-molecule real-time sequencing, high-throughput chromatin conformation capture mapping, and Illumina short-read sequencing.

Findings

We generated a 1.11-Gb goose genome with contig and scaffold N50 values of 1.85 and 33.12 Mb, respectively. The assembly contains

SUBMITTER: Li Y 

PROVIDER: S-EPMC7585555 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature