Mitochondrial Dysfunction and Morphological Abnormality in Keloid Fibroblasts

ABSTRACT: Objective: As an aberrant response to cutaneous wound healing, keloid scarring is a challenge to cure, and its clinical management remains unsatisfactory. The purpose of this study was to investigate whether mitochondrial function and morphology were impaired in keloid pathology. Approach: Keloid fibroblasts (KFs) and normal skin fibroblasts (NFs) were isolated and cultured from excised samples. A real-time extracellular flux analyzer was used to monitor the oxygen consumption rate for evaluating the mitochondrial respiratory function. Real-time PCR or Western blot was performed to detect the levels of mRNAs or proteins, respectively, including those associated with the tricarboxylic acid cycle, mitochondrial fission and fusion, and mitochondrial biogenesis. The reactive oxygen species (RO

SUBMITTER: Li Q 

PROVIDER: S-EPMC7585624 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature