Sustained Stimulation of β₂AR Inhibits Insulin Signaling in H9C2 Cardiomyoblast Cells Through the PKA-Dependent Signaling Pathway.

ABSTRACT:

Introduction

This study aimed to investigate the role of β₂ adrenergic receptor (β₂AR) in insulin signaling transduction in H9C2 cardiomyoblast cells to understand the formation of the β₂AR-insulin receptor (IR) protein complex and its role in insulin-induced Glut4 expression.

Methods

H9C2 cells were treated with various protein inhibitors (CGP, β₁AR inhibitor CGP20712; ICI, β₂AR inhibitor ICI 118,551; PKI, PKA inhibitor myristoylated PKI; PD 0325901, MEK inhibitor; SP600125, JNK inhibitor) with or without insulin or isoproterenol (ISO) before RNA-sequencing (RNA-Seq) and quantitative-PCR (Q-PCR). Yeast two-hybrid, co-immunoprecipitation and His-tag pull-down assay were carried out to investigate the formation of

SUBMITTER: Pei J 

PROVIDER: S-EPMC7585860 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature