Live Attenuated Measles Virus Vaccine Expressing Helicobacter pylori Heat Shock Protein A.

ABSTRACT: Measles virus (MV) Edmonston derivative strains are attractive vector platforms in vaccine development and oncolytic virotherapy. Helicobacter pylori heat shock protein A (HspA) is a bacterial heat shock chaperone with essential function as a Ni-ion scavenging protein. We generated and characterized the immunogenicity of an attenuated MV strain encoding the HspA transgene (MV-HspA). MV-HspA showed faster replication within 48 h of infection with >10-fold higher titers and faster accumulation of the MV proteins. It also demonstrated a superior tumor-killing effect in vitro against a variety of human solid tumor cell lines, including sarcoma, ovarian and breast cancer. Two intraperitoneal (i.p.) doses of 10⁶ 50% tissue culture infectious dose (TCID₅₀) MV-H

SUBMITTER: Iankov ID 

PROVIDER: S-EPMC7585873 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature