Endothelial prostaglandin D₂ opposes angiotensin II contractions in mouse isolated perfused intracerebral microarterioles.

ABSTRACT:

Hypothesis

A lack of contraction of cerebral microarterioles to Ang II ("resilience") depends on cyclooxygenase (COX) and lipocalin type prostaglandin D sythase L-PGDS producing PGD₂ that activates prostaglandin D type 1 receptors (DP1Rs) and nitric oxide synthase (NOS).

Materials & methods

Contractions were assessed in isolated, perfused vessels and NO by fluorescence microscopy.

Results

The mRNAs of penetrating intraparenchymal cerebral microarterioles versus renal afferent arterioles were >3000-fold greater for L-PGDS and DP1R and 5-fold for NOS III and COX 2. Larger cerebral arteries contracted with Ang II. However, cerebral microarterioles were entirely unresponsive but contracted with endothelin 1 and perfusion pressure. Ang II contractions were evoke

SUBMITTER: Li L 

PROVIDER: S-EPMC7585895 | biostudies-literature | 2020 Oct-Dec

REPOSITORIES: biostudies-literature