Long-term single-cell passaging of human iPSC fully supports pluripotency and high-efficient trilineage differentiation capacity.

ABSTRACT:

Objectives

To establish a straightforward single-cell passaging cultivation method that enables high-quality maintenance of human induced pluripotent stem cells without the appearance of karyotypic abnormalities or loss of pluripotency.

Methods

Cells were kept in culture for over 50 passages, following a structured chronogram of passage and monitoring cell growth by population doubling time calculation and cell confluence. Standard procedures for human induced pluripotent stem cells monitoring as embryonic body formation, karyotyping and pluripotency markers expression were evaluated in order to assess the cellular state in long-term culture. Cells that underwent these tests were then subjected to differentiation into keratinocytes, cardiomyocytes and definitive endoderm to

SUBMITTER: Cruvinel E 

PROVIDER: S-EPMC7586033 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature