Evolutionary selection of a 19-stranded mitochondrial β-barrel scaffold bears structural and functional significance.

ABSTRACT: Transmembrane β-barrels of eukaryotic outer mitochondrial membranes (OMMs) are major channels of communication between the cytosol and mitochondria and are indispensable for cellular homeostasis. A structurally intriguing exception to all known transmembrane β-barrels is the unique odd-stranded, i.e. 19-stranded, structures found solely in the OMM. The molecular origins of this 19-stranded structure and its associated functional significance are unclear. In humans, the most abundant OMM transporter is the voltage-dependent anion channel. Here, using the human voltage-dependent anion channel as our template scaffold, we designed and engineered odd- and even-stranded structures of smaller (V2¹⁶, V2¹⁷, V2¹⁸) and larger (V2²⁰, V2²¹)

SUBMITTER: Srivastava SR 

PROVIDER: S-EPMC7586230 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature