Development of novel parameter for monitoring of malignant melanoma progression.

ABSTRACT:

Objective

Increasing HIFs in malignant melanoma, the highly aggressive skin tumour, results in the stimulation of invasiveness. Increased HIF-1α fallouts in inhibition of the activity of some mitochondrial enzymes and leads to preference of cytosol energetic metabolism. Increase of aerobic glycolysis is reflected in an increase of free NADH (Warburg effect) and develops the malignant melanoma.Our goal was to find a link between hypoxia, or hypoxia mimicking factors and the stage of malignant melanoma. Furthermore, we focused on the finding of the experimental parameter which could monitor melanoma patients.

Patients and methods

We targeted HIF-1α gene expression and VDR rs2107301 gene polymorphism by PCR analysis. We detected the level of NADH in blood plasma by fluorescence

SUBMITTER: Spakova I 

PROVIDER: S-EPMC7586240 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature