Multimodal single-cell analysis reveals distinct radioresistant stem-like and progenitor cell populations in murine glioma.

ABSTRACT: Radiation therapy is part of the standard of care for gliomas and kills a subset of tumor cells, while also altering the tumor microenvironment. Tumor cells with stem-like properties preferentially survive radiation and give rise to glioma recurrence. Various techniques for enriching and quantifying cells with stem-like properties have been used, including the fluorescence activated cell sorting (FACS)-based side population (SP) assay, which is a functional assay that enriches for stem-like tumor cells. In these analyses, mouse models of glioma have been used to understand the biology of this disease and therapeutic responses, including the radiation response. We present combined SP analysis and single-cell RNA sequencing of genetically-engineered mouse models of glioma to show a time cour

SUBMITTER: Alexander J 

PROVIDER: S-EPMC7586969 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature