Oligonucleotide hybridization with magnetic separation assay for multiple SNP phasing.

ABSTRACT: Since humans have two copies of each gene, multiple mutations in different loci may or may not be found on the same strand of DNA (i.e., inherited from one parent). When a person is heterozygous at more than one position, the placement of these mutations, also called the haplotype phase, (i.e., cis for the same strand and trans for different strands) can result in the expression of different amount and type of proteins. In this work, we described an enzyme-free method to phase two single nucleotide polymorphisms (SNPs) using two fluorophore/quencher-labelled probes, where one of which was biotinylated. The fluorescence signal was obtained twice: first, after the addition of the labelled probes and second, after the addition of the magnetic beads. The first signal was shown to

SUBMITTER: Lee Yu HL 

PROVIDER: S-EPMC7587028 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature