Selective pressurized liquid extraction of plant secondary metabolites: Convallaria majalis L. as a case.

ABSTRACT: A fast and efficient selective pressurized liquid extraction (sPLE) method was developed to extract secondary metabolites from complex plant matrix. Convallaria majalis L., a plant producing toxic steroids, was used as proof-of-concept. The method was optimized in the aspects of preheating, dispersant, extraction temperature and solvent, and the use of C18 as in-cell cleanup sorbent. Eight authentic natural steroids with diverse sugar moieties and hydrophobicities were selected as reference analytes and spiked to 0.1 g dried leaves. The extraction performance was evaluated based on the analytes' stability, recovery, matrix effect in the electrospray interface and the level of co-extractives. With the optimal method, the extraction was finished in 10 min. A colorless extract was obta

SUBMITTER: Liang X 

PROVIDER: S-EPMC7587049 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature