Aneuploidy-associated gene expression signatures characterize malignant transformation in ulcerative colitis.

ABSTRACT:

Background

Malignant transformation in ulcerative colitis (UC) is associated with pronounced chromosomal instability, reflected by aneuploidy. Although aneuploidy can precede primary cancer diagnosis in UC for more than a decade, little is known of its cellular consequences.

Methods

Whole-genome gene expression analysis was applied to noninflamed colon mucosa, mucosal biopsies of patients with UC, and UC-associated carcinomas (UCCs). DNA image cytometry was used to stratify samples into ploidy types. Differentially expressed genes (DEGs) were analyzed by Ingenuity Pathway Analysis and validated by real-time quantitative PCR.

Results

Gene expression changes were more pronounced between normal mucosa and UC (2587 DEGs) than between UC and UCC (827 DEGs). Cytometry ident

SUBMITTER: Gerling M 

PROVIDER: S-EPMC7587454 | biostudies-literature | 2013 Mar-Apr

REPOSITORIES: biostudies-literature