Real-time deformability cytometry reveals sequential contraction and expansion during neutrophil priming.

ABSTRACT: It has become increasingly apparent that the biomechanical properties of neutrophils impact on their trafficking through the circulation and in particularly through the pulmonary capillary bed. The retention of polarized or shape-changed neutrophils in the lungs was recently proposed to contribute to acute respiratory distress syndrome pathogenesis. Accordingly, this study tested the hypothesis that neutrophil priming is coupled to morpho-rheological (MORE) changes capable of altering cell function. We employ real-time deformability cytometry (RT-DC), a recently developed, rapid, and sensitive way to assess the distribution of size, shape, and deformability of thousands of cells within seconds. During RT-DC analysis, neutrophils can be easily identified within anticoagulated "whole blood"

SUBMITTER: Bashant KR 

PROVIDER: S-EPMC7587463 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature