Isolation and Structure Determination of Echinochrome A Oxidative Degradation Products.

ABSTRACT: Echinochrome A (Ech A, 1) is one of the main pigments of several sea urchin species and is registered in the Russian pharmacopeia as an active drug substance (Histochrome^®), used in the fields of cardiology and ophthalmology. In this study, Ech A degradation products formed during oxidation by O₂ in air-equilibrated aqueous solutions were identified, isolated, and structurally characterized. An HPLC method coupled with diode-array detection (DAD) and mass spectrometry (MS) was developed and validated to monitor the Ech A degradation process and identify the appearing compounds. Five primary oxidation products were detected and their structures were proposed on the basis of high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) as 7-ethyl-2,2,3,3,

SUBMITTER: Mishchenko NP 

PROVIDER: S-EPMC7587531 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature