Research Note: Development of an ELISA to distinguish between goose parvovirus infection and vaccine immunization antibodies.

ABSTRACT: Goose parvovirus (GPV) leads to a huge loss in the poultry industry, and early diagnosis is required to prevent the disease from spreading. At present, there are a variety of detection methods for GPV infection, and the ELISA method has the advantages of simple and rapid operation. However, most ELISA methods for detecting GPV can only detect the antibody level of the sample, but cannot distinguish between the GPV infection and vaccine immunization antibodies. Therefore, this study has a wider application value by establishing the detection method based on the structure and non-structural protein of the virus. The GPV non-structural (NS1) and structure (VP3) fusion proteins were used as coating antigens to establish 2 indirect ELISA methods, and the detection conditions were optimized. A s

SUBMITTER: Zhang S 

PROVIDER: S-EPMC7587739 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature