Copper Transporters? Glutathione Reactivity of Products of Cu-Aβ Digestion by Neprilysin.

ABSTRACT: Aβ_4-42 is the major subspecies of Aβ peptides characterized by avid Cu(II) binding via the ATCUN/NTS motif. It is thought to be produced in vivo proteolytically by neprilysin, but in vitro experiments in the presence of Cu(II) ions indicated preferable formation of C-terminally truncated ATCUN/NTS species including Cu^IIAβ_4-16, Cu^IIAβ_4-9, and also Cu^IIAβ_12-16, all with nearly femtomolar affinities at neutral pH. Such small complexes may serve as shuttles for copper clearance from extracellular brain spaces, on condition they could survive intracellular conditions upon crossing biological barriers. In order to ascertain such possibility, we studied the reactions of Cu^IIAβ_4-16, Cu<

SUBMITTER: Stefaniak E 

PROVIDER: S-EPMC7588031 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature