A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling.

ABSTRACT: G protein-coupled receptors (GPCRs) allow cells to respond to chemical and sensory stimuli through generation of second messengers, such as cyclic AMP (cAMP), which in turn mediate a myriad of processes, including cell survival, proliferation, and differentiation. In order to gain deeper insights into the complex biology and physiology of these key cellular pathways, it is critical to be able to globally map the molecular factors that shape cascade function. Yet, to this date, efforts to systematically identify regulators of GPCR/cAMP signaling have been lacking. Here, we combined genome-wide screening based on CRISPR interference with a novel sortable transcriptional reporter that provides robust readout for cAMP signaling, and carried out a functional screen for regulators of the pathway

SUBMITTER: Semesta KM 

PROVIDER: S-EPMC7588078 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature