P-type Na⁺/K⁺ ATPases essential and nonessential for cellular homeostasis and insect pathogenicity of Beauveria bassiana.

ABSTRACT: ENA1 and ENA2 are P-type IID/ENA Na⁺/K⁺-ATPases required for cellular homeostasis in yeasts but remain poorly understood in filamentous fungal insect pathogens. Here, we characterized seven genes encoding five ENA1/2 homologues (ENA1a-c and ENA2a/b) and two P-type IIC/NK Na⁺/K⁺-ATPases (NK1/2) in Beauveria bassiana, an insect-pathogenic fungus serving as a main source of fungal insecticides worldwide. Most of these genes were highly responsive to alkaline pH and Na⁺/K⁺ cues at transcription level. Cellular Na⁺, K⁺ and H⁺ homeostasis was disturbed only in the absence of ena1a or ena2b. The disturbed homeostasis featured acceleration of vacuolar acidification, elevation of

SUBMITTER: Mou YN 

PROVIDER: S-EPMC7588218 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature