Mesoscopic 3D imaging of pancreatic cancer and Langerhans islets based on tissue autofluorescence.

ABSTRACT: The possibility to assess pancreatic anatomy with microscopic resolution in three dimensions (3D) would significantly add to pathological analyses of disease processes. Pancreatic ductal adenocarcinoma (PDAC) has a bleak prognosis with over 90% of the patients dying within 5 years after diagnosis. Cure can be achieved by surgical resection, but the efficiency remains drearily low. Here we demonstrate a method that without prior immunohistochemical labelling provides insight into the 3D microenvironment and spread of PDAC and premalignant cysts in intact surgical biopsies. The method is based solely on the autofluorescent properties of the investigated tissues using optical projection tomography and/or light-sheet fluorescence microscopy. It does not interfere with subsequent histopathologi

SUBMITTER: Hahn M 

PROVIDER: S-EPMC7588461 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature