Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle.

ABSTRACT: Methanol is a biotechnologically promising substitute for food and feed substrates since it can be produced renewably from electricity, water and CO₂. Although progress has been made towards establishing Escherichia coli as a platform organism for methanol conversion via the energy efficient ribulose monophosphate (RuMP) cycle, engineering strains that rely solely on methanol as a carbon source remains challenging. Here, we apply flux balance analysis to comprehensively identify methanol-dependent strains with high potential for adaptive laboratory evolution. We further investigate two out of 1200 candidate strains, one with a deletion of fructose-1,6-bisphosphatase (fbp) and another with triosephosphate isomerase (tpiA) deleted. In contrast to previous reported methanol-depende

SUBMITTER: Keller P 

PROVIDER: S-EPMC7588473 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature