EV-origin: Enumerating the tissue-cellular origin of circulating extracellular vesicles using exLR profile.

ABSTRACT: Extracellular vesicles (EVs) are complex ecosystems that can be derived from all body cells and circulated in the body fluids. Characterizing the tissue-cellular source contributing to circulating EVs provides biological information about the cell or tissue of origin and their functional states. However, the relative proportion of tissue-cellular origin of circulating EVs in body fluid has not been thoroughly characterized. Here, we developed an approach for digital EVs quantification, called EV-origin, that enables enumerating of EVs tissue-cellular source contribution from plasma extracellular vesicles long RNA sequencing profiles. EV-origin was constructed by the input matrix of gene expression signatures and robust deconvolution algorithm, collectively used to separate the relative pro

SUBMITTER: Li Y 

PROVIDER: S-EPMC7588739 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature