Using chemiluminescence imaging of cells (CLIC) for relative protein quantification.

ABSTRACT: Cell physiology and cellular responses to external stimuli are partly controlled through protein binding, localization, and expression level. Thus, quantification of these processes is pivotal in understanding cellular biology and disease pathophysiology. However, it can be methodologically challenging. Immunofluorescence is a powerful technique, yet quantification by this method can be hampered by auto-fluorescence. Here we describe a simple, sensitive and robust chemiluminescence-based immunoassay (chemiluminescence imaging of cells; CLIC) for relative quantification of proteins. We first employed this method to quantify complement activation in cultured mammalian cells, and to quantify membrane protein expression, shedding, binding and internalization. Moreover, through specific membran

SUBMITTER: Fisher J 

PROVIDER: S-EPMC7589485 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature