Deep phenotypic characterization of immunization-induced antibacterial IgG repertoires in mice using a single-antibody bioassay.

ABSTRACT: Antibodies with antibacterial activity need to bind to the bacterial surface with affinity, specificity, and sufficient density to induce efficient elimination. To characterize the anti-bacterial antibody repertoire, we developed an in-droplet bioassay with single-antibody resolution. The assay not only allowed us to identify whether the secreted antibodies recognized a bacterial surface antigen, but also to estimate the apparent dissociation constant (K_{D app}) of the interaction and the density of the recognized epitope on the bacteria. Herein, we found substantial differences within the K_{D app}/epitope density profiles in mice immunized with various species of heat-killed bacteria. The experiments further revealed a high cross-reactivity of the secreted IgG repertoire

SUBMITTER: Heo M 

PROVIDER: S-EPMC7589517 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature