Discovery of New Antibacterial Accramycins from a Genetic Variant of the Soil Bacterium, <i>Streptomyces</i> sp. MA37.

ABSTRACT: Continued mining of natural products from the strain Streptomyces sp. MA37 in our laboratory led to the discovery of a minor specialized metabolite (SM) called accramycin A. Owing to its low yield (0.2 mg/L) in the wild type strain, we investigated the roles of regulatory genes in the corresponding biosynthetic gene cluster (acc BGC) through gene inactivation with the aim of improving the titer of this compound. One of the resulting mutants (∆accJ) dramatically upregulated the production of accramycin A 1 by 330-fold (66 mg/L). Furthermore, ten new metabolites, accramycins B-K 2-11, were discovered, together with two known compounds, naphthacemycin B₁12 and fasamycin C 13 from the mutant extract. This suggested that accJ

SUBMITTER: Maglangit F 

PROVIDER: S-EPMC7590149 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature