Culex pipiens and Culex restuans egg rafts harbor diverse bacterial communities compared to their midgut tissues.

ABSTRACT:

Background

The bacterial communities associated with mosquito eggs are an essential component of the mosquito microbiota, yet there are few studies characterizing and comparing the microbiota of mosquito eggs to other host tissues.

Methods

We sampled gravid female Culex pipiens L. and Culex restuans Theobald from the field, allowed them to oviposit in the laboratory, and characterized the bacterial communities associated with their egg rafts and midguts for comparison through MiSeq sequencing of the 16S rRNA gene.

Results

Bacterial richness was higher in egg rafts than in midguts for both species, and higher in Cx pipiens than Cx. restuans. The midgut samples of Cx. pipiens and Cx. restuans were dominated by Providencia. Culex pipiens and Cx. restuans egg rafts sample

SUBMITTER: Juma EO 

PROVIDER: S-EPMC7590256 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature