Prostasin and hepatocyte growth factor B in factor VIIa generation: Serine protease knockdowns in zebrafish.

ABSTRACT:

Background

Blood clotting in humans is initiated by the binding of tissue factor to activated coagulation factor VII (FVIIa) in the plasma. Previous studies have reported that hepsin and factor VII (FVII)-activating protease are responsible for generating FVIIa.

Objectives

We aimed to identify other proteases that may activate FVII using zebrafish as a model.

Methods

We screened 179 genes encoding serine protease domains using the piggyback knockdown method to identify genes involved in the activation of zebrafish Fvii. A prolonged kinetic prothrombin time (kPT) assay was used to detect gene knockdown effects.

Results

In the primary screen, 21 genes showed prolonged kPT. In the secondary screen, 14 of 21 genes showed positive results. In the tertiary screen, al

SUBMITTER: Khandekar G 

PROVIDER: S-EPMC7590325 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature