MiR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1.

ABSTRACT:

Introduction

Prostate cancer (PCa) is one of the most common types of cancer in men. In the course of the development and progression of this disease, abnormal expression of miR-203 is usually accompanied. However, its role in prostate tumorigenesis and the underlying mechanism are poorly understood.

Methods

Dual luciferase reporter gene analysis was used to detect miR-203 binding site in insulin receptor substrates 1 (IRS-1). Cell proliferation was assessed by MTT assay in PCa cells with either IRS-1 knockdown or miR-203 overexpression. IRS-1 and other proteins expression in PCa cells was assessed by Western Blot.

Results

we found that the insulin receptor substrates 1 (IRS-1) is a novel target of miR-203 in PCa and miR-203 can specifically bind to the 3'UTR region o

SUBMITTER: Meng Y 

PROVIDER: S-EPMC7590475 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature