Quantifying Liver Size in Larval Zebrafish Using Brightfield Microscopy.

ABSTRACT: In several transgenic zebrafish models of hepatocellular carcinoma (HCC), hepatomegaly can be observed during early larval stages. Quantifying larval liver size in zebrafish HCC models provides a means to rapidly assess the effects of drugs and other manipulations on an oncogene-related phenotype. Here we show how to fix zebrafish larvae, dissect the tissues surrounding the liver, photograph livers using bright-field microscopy, measure liver area, and analyze results. This protocol enables rapid, precise quantification of liver size. As this method involves measuring liver area, it may underestimate differences in liver volume, and complementary methodologies are required to differentiate between changes in cell size and changes in cell number. The dissection technique described herein is

SUBMITTER: Kotiyal S 

PROVIDER: S-EPMC7590575 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature