Performance evaluation of five ELISA kits for detecting anti-SARS-COV-2 IgG antibodies.

ABSTRACT:

Objectives

To evaluate and compare the performances of five commercial ELISA assays (EDI, AnshLabs, Dia.Pro, NovaTec, and Lionex) for detecting anti-SARS-CoV-2 IgG.

Methods

Seventy negative control samples (collected before the COVID-19 pandemic) and samples from 101 RT-PCR-confirmed SARS-CoV-2 patients (collected at different time points from symptom onset: ≤7, 8-14 and >14 days) were used to compare the sensitivity, specificity, agreement, and positive and negative predictive values of each assay with RT-PCR. A concordance assessment between the five assays was also conducted. Cross-reactivity with other HCoV, non-HCoV respiratory viruses, non-respiratory viruses, and nuclear antigens was investigated.

Results

Lionex showed the highest specificity (98.6%; 95% CI 92.

SUBMITTER: Yassine HM 

PROVIDER: S-EPMC7590641 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature